Brucella – Serology
|Test Requested||Required Requisition(s)||Specimen Type||Minimum Volume||Collection Kit|
Whole blood or serum
5.0 ml blood or 1.0 ml serum
Vacutainer tubes (SST)
Submission and Collection Notes
Indicate the following on the General Test Requisition Form if diagnostic testing is requested:
- Clinical symptoms
- Exposure (e.g. consumption of unpasteurized dairy products)
- Travel history
- Onset date
Timing of Specimen Collection
An acute (collected early after the onset of symptoms) and a convalescent (collected 2-3 weeks later) may be required for laboratory diagnosis.
Haemolysed, icteric, lipemic or microbially contaminated sera or plasma are not recommended for testing.
Storage and Transport
Label the specimen container with the patient’s full name, date of collection and one other unique identifier such as the patient’s date of birth or Health Card Number. Failure to provide this information may result in rejection or testing delay.
Centrifuge if using SST. Label specimens with at least two identifiers; place it in biohazard bag and seal. Specimens should be stored at 2-8°C following collection and shipped to PHOL on ice packs.
Test Frequency and Turnaround Time (TAT)
Brucella serology testing is performed once per week.
Turnaround time is up to 10 days from receipt by PHO Laboratory.
Brucella serology testing is performed using Serum Tube Agglutination (STA) assay.
Results are reported to the ordering physician or health care provider as indicated on the requisition.
Specimens that are positive for Brucellosis are reported to the Medical Officer of Health as per Health Protection and Promotion Act.
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